| | Frequency | Notes | |---|---|---| | External cleaning | Weekly | Use a soft, lint‑free cloth lightly dampened with 70% ethanol. Do not spray liquids directly onto the instrument. | | Lamp check | Monthly | Run a background scan with an empty plate. If the signal drops >20% from the baseline, the lamp may need replacement. | | Absorbance calibration | Annually (or after lamp change) | Use a certified absorbance standard plate (e.g., NIST‑traceable). The Magellan software includes a calibration wizard. | | Alignment verification | Biannually | For fluorescence polarization assays, run a known polarizing standard to ensure the polarizers are correctly oriented. |
Features 16 selectable bandwidth settings. Standard ranges are 2.5 nm to 10 nm for UV (below 280 nm) and 5 nm to 20 nm for visible light. Plate Formats: Compatible with 6-well to 1,536-well microplates. Measurement Modes: tecan safire 2 manual pdf
Set the photomultiplier tube (PMT) gain. For unknown sample concentrations, use the "Optimal Gain" function, which flashes a designated well to calculate a dynamic range that avoids signal saturation. 3. Executing the Run | | Frequency | Notes | |---|---|---| |
Optimizing the gain and integration time to maximize sensitivity without saturation. 4. Maintenance and Troubleshooting If the signal drops >20% from the baseline,
Check lamp usage hours in software diagnostics; contact service for lamp replacement. Mechanical obstruction or dirty guide rails.
Once you secure the PDF, knowing its structure will save you hours of frustration. A complete manual typically contains the following sections:
Proper installation ensures data integrity and prolongs the lifespan of the optical components.